Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma
نویسندگان
چکیده
A high performance liquid chromatographic method for determination of rifabutin in human plasma was developed. The method involved deproteinisation of the sample with acetonitrile and analysis of the supernatant using a reversed-phase C18 column (250mm) and UV detection at a wavelength of 265nm. The assay was specifi c for rifabutin and linear from 0.025 to 10.0μg/ml. The relative standard deviation of intraand inter-day assays was lower than 10%. The method was able to remove interfering materials in plasma, yielding an average recovery of rifabutin from plasma of 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies of rifabutin.
منابع مشابه
Simple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma
A rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. Ofloxacin was used as an internal standard and separation carried on a Novapak C18 column using a mobile phase of 0.01 M phosphate buffer (pH =2.6): methanol (82: 18 v/v). Extraction of drug was performed from plasma by liquid-...
متن کاملSimple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma
A rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. Ofloxacin was used as an internal standard and separation carried on a Novapak C18 column using a mobile phase of 0.01 M phosphate buffer (pH =2.6): methanol (82: 18 v/v). Extraction of drug was performed from plasma by liquid-...
متن کاملA Rapid, Simple, Liquid Chromatographic-Electrospray Ionization, Ion Trap Mass Spectrometry Method for the Determination of Finasteride in Human Plasma and its Application to Pharmacokinetic Study
A fast, accurate, sensitive, selective and reliable method using reversed-phase high performance liquid chromatography coupled to electrospray ionization ion trap mass spectrometry was developed and validated for the determination of finasteride in human plasma. After protein precipitation with perchloric acid, satisfactory separation was achieved on a Zorbax Eclipse® C8 analytical column u...
متن کاملA Rapid, Simple, Liquid Chromatographic-Electrospray Ionization, Ion Trap Mass Spectrometry Method for the Determination of Finasteride in Human Plasma and its Application to Pharmacokinetic Study
A fast, accurate, sensitive, selective and reliable method using reversed-phase high performance liquid chromatography coupled to electrospray ionization ion trap mass spectrometry was developed and validated for the determination of finasteride in human plasma. After protein precipitation with perchloric acid, satisfactory separation was achieved on a Zorbax Eclipse® C8 analytical column u...
متن کاملA simple, sensitive and rapid isocratic reversed-phase high-performance liquid chromatography method for determination and stability study of curcumin in pharmaceutical samples
Objective: This study was designed to develop and validate a new reversed-phase high-performance liquid chromatography (RP-HPLC) method based on Q2 (R1) International Conference on Harmonization (ICH) guideline for determination of curcumin in pharmaceutical samples. Materials and Methods: The HPLC instrument method was optimized with isocratic elution with acetonitrile: ammonium acetate (45:55...
متن کامل